Fig. 6. In vivo MT-binding dynamics of GFP-ensconsin chimeras are reduced by treatment with staurosporine. Fluorescent speckle microscopy (FSM) was used to record the dynamics of 5xGFP-EMTB chimeras in living cells. (A,B,C) Time-lapse micrographs of 5xGFP-EMTB speckles in living cells, treated with (A) staurosporine (25 nM, 2 hours), a general inhibitor of protein kinases; (B) no treatment; and (C) sodium azide and deoxyglucose (5 mM and 1 mM, 30 minutes) to substantially reduce cellular ATP. Arrows indicate speckles that remain from one panel to the next; open, white arrowheads denote speckles that disappear during the time-lapse sequence; and black-filled arrowheads denote speckles that appear during the time-lapse sequence. Elapsed time of each exposure is shown in seconds under each panel. (D-F) Kymographs of a MT segment from each of the time-lapse sequences; the scale markers on the left indicate the distance and time over which the kymographs were made. Kymographs and micrographs are the same magnification. Note that treatment with staurosporine substantially reduced dynamics of 5xGFP-EMTB speckles (A,D), in comparison with untreated cells (B,E).