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Fig. 1. Transfection of cultured HEK 293 cells. (A) A cell transfected with RFP. Diffuse RFP protein is observed in the cytoplasm and the nucleus but the staining in the latter is stronger. (B,C) Two examples of cells transfected with ac.CaP-RFP. These ac.CaP-RFP cells display striking morphological changes compared with RFP-transfected cells. (D) A cell transfected with ac.CaP{Delta}-GFP that does not have the actin-binding domains. The staining observed is diffuse in the cytoplasm and the nucleus. The ac.CaP{Delta}-GFP-transfected cells did not display any morphological change compared with RFP-transfected cells. Bar, 15 µm.