Fig. 5. Immunolocalization of MdrA1. Wild-type DH1 (A-D) and rtoA^- (E-H) cells were fixed and stained with anti-MdrA1 antibody raised against the GST-MdrA1 fusion protein (B, D, F, H). The corresponding phase images are shown in A, C, E and G. The cells were either taken directly from growth media (A, B, E, F) or osmotically stressed (C, D, G, H) before fixation. Bar, 10 µm.