Fig. 3. Expression of chagasin during the life cycle of T. cruzi. Western blotting of Triton X-100 lysates (10 µg protein) from the three developmental stages of Dm28c T. cruzi. The membranes were incubated with rab
chagasin (1:1000) in PBS, 2 mM EDTA, 3% (w/v) nonfat dried milk, 0.05% (v/v) Tween 20 for 1 hour at room temperature. Lane 1, r-chagasin as a reference (100 ng); lane 2, trypomastigotes; lane 3, epimastigotes; and lane 4, amastigotes.