Fig. 5. Screening of anti-chagasin antibodies in human sera. A panel of 25 sera from chronic chagasic patients, and 25 normal control human sera, were tested at 1:50 dilution against immobilized r-chagasin by ELISA. Following incubation with anti-human IgG conjugated to peroxidase, the color development was detected at 492 nm. Control wells coated with BSA and exposed to serum samples did not yield reactions (not shown).