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Fig. 7. Sub-cellular localization of chagasin. Trypomastigotes (A,B) displayed intense and moderate labeling in flagellar pocket (A) and cytoplasmic vesicles (B, arrow), respectively, but cell surface was poorly labeled (arrowheads). Intermediate forms (C) presented intensely labeled cell surface (arrowheads) as well as flagellar pocket. T. cruzi amastigote (D) showing intense immunolabeling at both cell surface (arrowheads) and flagellar pocket. Flagellar pocket (P), flagellum (F), kinetoplast (K) and nucleus (N).