Fig. 3. Cryosections of wild-type GHR cells co-incubated with BSA-5 nm gold and GH for 1 hour. (A-C) BSA-gold (5 nm) and GH (10 nm gold) co-localize extensively in late endosomes (LE). (B,D) Lysosomes (L) are reached by BSA gold but lack GH staining, which is probably due to rapid degradation. EE, early endosome; N, nucleus; P, plasma membrane. Scale bars: 200 nm.