Fig. 2. Immunolocalization studies with undifferentiated and differentiated muscle cells. C2C12 myoblasts (A) and myotubes (B) were fixed in methanol and labeled with antibodies to lamin A (LA-2H10, LA-2B3), lamin B1 (LB-P), RNA splicing factors (SC-35, U5-116 kDa) or myosin. (C) Unfixed, frozen transverse sections of adult skeletal muscle were stained with LA-2H10 or LA-2B3 (green) and counterstained with DAPI (red). A single myofiber is depicted in each panel. Scale bar: 10 µm.