Fig. 5. Localization of lamin A speckles and markers for myoblast differentiation. (A,B) C2C12 cells were kept in differentiation medium for 0-72 hours, fixed with formaldehyde and labeled with mAb LA-2H10 and myogenin antibody or LA-2H10 and myosin antibody. (C,D) C2C12 cells were maintained in differentiation medium for 6-24 hours, fixed with formaldehyde and labeled with LA-2H10 and myogenin antibody, or LA-2H10 and p21 antibody. (E,F) Quantitative analysis of data in C,D, with n=150 cells at each time point. Arrows in C indicate cells expressing myogenin but not lamin A speckles. Scale bar: 10 µm.