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Fig. 1. Co-localization of Stx1B receptors and CTB receptors GM1 on the apical surface of Caco-2 intestinal epithelial cells. Confocal optical section through the apical surface of Caco-2 monolayers. Cells were double labeled with Stx1B-Oregon Green 488 (green) and CTB-TMR (red). Overlay panel shows co-localization of both receptors (yellow). Note, not all cells in confluent Caco-2 monolayers bind CTB and Stx1B due to the cell heterogeneity.