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Fig. 1. Colorimetric BrdU cell proliferation ELISA of human corneal epithelial cells cultured under normoxic (20% O2) or hypoxic (2% O2) conditions. Proliferation is represented as the mean absorbance (A450) in arbitrary units + s.e.m. (n=30 for each time point and oxygen level). For 3, 7 and 10 days in culture, proliferation is significantly higher (*, P<0.0001) in normoxic HCE-T compared with hypoxic HCE-T.