Fig. 4. Western blot and densitometric analysis of laminin 5 2 chain protein content in normoxic and hypoxic human corneal epithelial cells. HCE-T were cultured for 3 days (A) or 7 days (B) under normoxic (N) or hypoxic (H) conditions. 15 µg of cell lysates were resolved using 7.5% SDS-PAGE and processed for western blot analysis using mAb D4B5 to the 2 chain. The unprocessed 155 kDa protein and processed 105 kDa and 80 kDa proteins are detected by immunoblot. The relative density values of each band are indicated (in arbitrary units/µg total protein) to the right of each immunoreactive protein. The total of the three densitometry readings is also indicated.