Fig. 4. Rab22aQ64L, the GTPase-deficient mutant, colocalizes not only with early and late endosomes, but also with lysosomes and autophagosomes. Lysosomes were loaded with Rh-HRP by a 1 hour uptake followed by a 1 hour chase (A-C). Acidic compartments were labeled with LysoTracker red (D-F). Autophagosomes were labeled with MDC (monodansylcadaverine) (G-I). Enlarged lysosomal and autophagosomal compartments were evident in cells overexpressing Rab22aQ64L (t) when compared with untransfected cells (u). The bars represent 7 µm.