JCS -- Chun et al. 114 (22): 4051 Figure IG10

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Fig. 10. Co-immunoprecipitation of HIF-1 ${alpha}$ Z and ARNT. pARNT plasmid was transfected into stable cell lines transfected with pcDNA and pHA-HIF-1 ${alpha}$ Z plasmids. After 4 hours of hypoxia, the cytosolic proteins were immunoprecipitated (IP) by rabbit anti-HA antibody ( ${alpha}$ -HA) or goat anti-ARNT antibody ( ${alpha}$ -ARNT). HA-HIF-1 ${alpha}$ Z protein and ARNT protein were identified by western blotting, and are indicated by arrows.