Fig. 8. Nuclear translocation of ARNT. ARNT protein was extracted from the nuclei and the cytosol of the stable transfectant pHIF-1Z cell line. The cells were subjected to normoxia or hypoxia (4 hours), and 5 µg of protein from nuclear extracts or 40 µg of protein from total cellular lysates were separated by SDS-PAGE followed by western blotting with goat anti-ARNT antibody.