Fig. 9. Effect of MPB-91 on confocal immunofluorescent labelling of CFTR in F508/G551D cystic fibrosis epithelial cells from nasal brushings. Images show CFTR immunofluorescence in green with the nucleus counterstained with propidium iodide (red). A-D are untreated cells; E and F are cells treated for 2 hours at 37°C with MPB-91 (250 µM). The bar graph shows quantification of the effect of MPB-91 on CFTR location. 80 cells were examined from each condition and categorized as having a distinct CFTR location of either perinuclear (PN), juxtanuclear (JN), more evenly distributed (ED) throughout the cell including the apical region, or predominantly apical (Apical) as described in the text. Open bars are untreated cells; filled bars are cells treated with MPB-91.