Fig. 3. Identification of the major mouse FimH binding urothelial plaque protein by mass spectrometry and cDNA cloning. The 24 kDa protein identified as the major FimH and bacterial binding protein of purified mouse urothelial plaque was gel-purified, microsequenced and cDNA-cloned. (A) Identification of the major mouse FimH-binding protein as uroplakin Ia. The cDNA-deduced amino acid sequence of the mouse (mou) FimH binding protein is aligned here with those of human (hum) and bovine (bov) uroplakin Ias. The predicted N-glycosylation sites and the putative transmembrane domains are shaded in gray and underlined, respectively. Asterisks indicate amino acids that vary among the species. (B) Sequencing of a major tryptic peptide using mass spectrometry. The sequence of this peptide was derived from the y series ions and is indicated on the top from right (N-terminal) to left (C-terminal).