Fig. 7. Identification of connexins. 10 µg of protein was loaded into each lane of a 12% SDS-PAGE. Following electrophoresis, proteins were transferred to a PVDF membrane, and mouse monoclonal antibodies were used to probe for connexins 50, 43, or 32, all of which were detected. Positive lysates were run for connexins 43 and 32 to confirm identification. Known markers are indicated by their molecular mass (kDa) along the left edge. Results are representative of four independent experiments.