Fig. 6. BP180 co-distributes with actinin-4 and actinin-1 at cell-cell contact sites in MCF-10A cells. MCF-10A cells were processed for double-labeling using antibodies against either actinin-4 (A) or actinin-1 (D) in combination with an antibody preparation against BP180 (B,E). BP180 co-localizes with actinin-4 at sites of cell-cell contact (arrows in A,B). This appears yellow in the overlay of these images (C). Actinin-1 and BP180 also co-distribute at cell-cell contact sites (arrows in D,E). The areas of co-localization appear yellow in the overlay of the images (F). There is no obvious actinin-4 or actinin-1 where BP180 is found along sites of cell-substrate interaction (B,E, long arrows). Bar, 10 µm.