Fig. 7. The fate of the product of actinin-4 transgenes in transfected MCF-10A cells. MCF-10A cells were transfected with constructs expressing full-length actinin-4 fused with a GFP-tag (A) or an HA-tag (C). In E, cells were transfected with a truncated actinin-4 (residues 1-813). At 24 hours after transfection, cells were processed for immunofluorescence microscopy. HA.11 antibody was used to visualize HA-tagged actinin-4 in transfected cells in C,E. Full-length actinin-4 is absent from the nucleus but instead distributes to sites of cell-cell contact as well as in the cytoplasm (arrows in A,C). By contrast, truncated actinin-4 localizes exclusively to the nucleus (E). B, D and F show phase contrast images of the cells. Bar, 10 µm.