JCS -- Trinkle-Mulcahy et al. 114 (23): 4219 Figure IG4

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Fig. 4. Dynamic relocalization of PP1 ${gamma}$ by a spatially distinct targeting subunit. Heterokaryons created by the fusion of transiently transfected mammalian cells in the presence of cyclohexamide demonstrate that ECFP-NIPP1 can retarget the nucleolar pool of EYFP-PP1 ${gamma}$ to nucleoplasmic speckles in both HEK293 (A and B) and HeLa (E and F) cells, whereas the NIPP1(V201K/F203K) mutant cannot (C and D, HEK293; G and H, HeLa). The dashed arrow in Panel E indicates a nucleus within the EYFP-PP1 ${gamma}$ /ECFP-NIPP1 HeLa cell heterokaryon that has a lower level of NIPP1 than its neighbors and shows no significant retargeting of PP1 ${gamma}$ , demonstrating that this is a concentration-dependent effect. Arrows indicate nuclear speckles, whereas arrowheads indicate nucleoli. Scale bars are 10 µM.