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Fig. 8. Myofibril content versus days in culture. The open symbols show results for cardiomyocytes transfected with GFP-N-RAP (A), GFP-N-RAP-LIM (B), GFP-N-RAP-SR (C) and GFP-N-RAP-IB (D); in each case the results from cardiomyocytes transfected with GFP alone are shown for comparison (filled symbols). Cells were transfected after 1 day (circles) or 2 days (squares) in culture and viewed 1 day or 3 days post-transfection. Significant differences (P<0.05) between the N-RAP constructs and GFP alone were determined by unpaired Student’s t-tests and are indicated by asterisks. Each point represents the mean±s.e.m. from 3 to 40 transfected cells.