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Fig. 2. Cyclin B mRNA becomes undetectable after cells are treated with cyclin B dsRNA at the time of imbibition. (a) Cells treated with cyclin B dsRNA at the time of imbibition. (b) Untreated control cells. DIC images of cells assayed by in situ hybridization shows that the cyclin B mRNA is abundant 30 minutes after imbibition, but that it is undetectable after RNAi treatments. Bar, 25 µm.