Fig. 1. Regulation of hDlg protein by the proteasome in CaSKi cells. HPV-16-positive CaSKi cervical tumour cells were treated with proteasome inhibitors lactacystin (LC), N-CBZ-Leu-Leu-Leu-al (CBZ) and N-acetyl-Leu-Leu-norleucinal (LL), or with DMSO as control (–) for two hours before harvesting. Equal amounts of protein extracts were then separated by PAGE and analysed by western blot with -Dlg antiserum. In vitro translated Dlg (IVT Dlg) was included as a positive control and standard molecular weight protein markers (Celbio) are shown.