Fig. 2. CrPRF is the only profilin-like gene in Chlamydomonas. (A) Southern blot analysis of 10 µg wild-type genomic DNA digested by BamHI (B), EcoRI (E), SalI (S) or XhoI (X) revealed fragments of the predicted sizes, suggestive of a single CrPRF gene. (B) Northern blot analysis of 10 µg poly(A)⁺ mRNA from wild-type cells (NR) or from wild-type cells collected 30 minutes after deflagellation (R). Notably, the message increased dramatically following deflagellation. The message for the Chlamydomonas CRY gene (Yang and Sale, 1998) was used as a loading control in all northern blots (not shown).