Fig. 1. Ena/VASP proteins and Fyb/SLAP accumulate at sites of phagocytosis in Raw264.7 macrophages. (A,C) Analysis by western blotting of protein extracts of Raw264.7 macrophages using an anti-Evl monoclonal antibody (A) and an anti-Fyb/SLAP affinity-purified polyclonal antibody (C). (B,D) Localisation of Evl and Fyb/SLAP in Raw264.7 macrophages during phagocytosis. Raw264.7 cells were incubated with IgG-opsonised sheep RBC (for Evl labelling) or IgG-coated beads (for Fyb/SLAP labelling) for approximately five minutes, fixed and stained with the above mentioned antibodies. Both Evl (B, left pa nel) and Fyb/SLAP (D, left panel) localise to phagocytic cups where they colocalise with actin (B,D; right panels). Arrows point to examples of phagocytic cups surrounding sheep RBC (B) or beads (D). Scale bar: 10 µm.