Fig. 7. Influence of ActA repeats on the rate of phagocytosis. Raw264.7 cells were transiently transfected with wild-type or mutated GFP-tagged ActA repeats and then incubated with antibody-coated sheep RBC. After incubation at 37°C for 15 minutes, non-internalised sheep RBC were lysed with distilled water and the cells fixed. The phagocytic efficiency is expressed as the number of internalised sheep RBC per macrophage. Error bars indicate one standard deviation from the mean.