Fig. 9. Localisation of signalling proteins to phagocytic cups. Raw264.7 cells were incubated with antibody-coated sheep RBC, fixed and co-stained with Texas Red-conjugated phalloidin (A, C) and with antibodies against Nck (A'), Vav (B') or SLP-76 (C'). The three signalling proteins all localise to the phagocytic cups (arrowheads in A’, C’) where they colocalise with actin (arrowheads in A, C). Scale bar: 5 µm.