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Fig. 5. Plasmolysis disrupts premetaphase alignment. Treated zygotes were transferred to ASW containing either 0.6 or 0.7 M sucrose at 15 hours AF. (A) Alignment angles. Data points are the means±s.d. of three experiments. (B) The distance between the centrosomes at telophase; bars are the means±s.d. of two experiments.