Fig. 6. Centrosomal alignment and separation in Brefeldin A. (A) Centrosomal alignment angles were measured before transfer to Brefeldin A (at 15.5 hours AF) and again during mitosis (21.5 hours AF) on either untreated zygotes or on zygotes incubated in 5 µg/ml Brefeldin A. Data points are the means±s.d. of two experiments. (B) The distances between centrosomes at telophase were measured on zygotes cultured in 5 µg/ml Brefeldin A from 9 hours AF and bars indicate the means±s.d of three experiments.