Fig. 7. Effects of externally applied proteases. Treated zygotes were incubated in 0.1% (w/v) thermolysin and 0.1% (w/v) proteinase K beginning 15-17 hours AF. (A) Centrosomal alignment angles were measured on zygotes prior to treatment and during mitosis. (B) Distances between centrosomes at telophase. Data points in A and bars in B are the means±s.d of three experiments. Premetaphase (C) and telophase (D) microtubule arrays in protease-treated zygotes. Scale bar in C also applies to D.