Fig. 9. Centrosomal alignment in olomoucine. (A) Alignment angles were measured on four populations of either untreated zygotes progressing through mitosis or zygotes incubated in 35 µM olomoucine beginning 5 hours AF. In two of the populations, zygotes were released from olomoucine 24 hours AF and alignments of the centrosomal axes were measured on premetaphase and metaphase zygotes. Data points are means±s.d. (B) An elongated nucleus in an untreated zygote 16 hours AF. (C,D) Nuclei in olomoucine-treated zygotes 25.5 hours AF are also elongated. (E) An oval nucleus in a zygote cultured in olomoucine from 5 to 15.5 hours AF and then olomoucine and 30 nM latrunculin B from 15.5 hours AF until 39.5 hours AF. Scale bar in B also applies to C-E.