Fig. 10. Cut7 and Ark1.PkC do not show appreciable co-localisation as the spindle elongates during anaphase B. IH2035 cells were grown to mid-log phase in rich medium at 30°C and processed for Cut7 and Ark1.PkC staining. Cut7 staining of the termini of the metaphase spindle does not show a strong overlap with Ark1.PkC staining in the same cells (A). Similarly the distribution of the two molecules along the anaphase B spindle is very different (B,C). The restricted nature of the co-localisation of the two signals is graphically shown by the predominance of green and red signals over the co-localisation yellow signals in the merged image.