Fig. 8. Ark1.PkC and Nuf2.GFP staining as cells transit through the cell cycle. IH2035 was grown to mid-log phase before preparation for anti-Pk immunofluorescence microscopy. In each case the panels show (from right to left): DAPI/DIC, DAPI, Nuf2.GFP fluorescence, Ark1.PkC staining and a merge of the two middle panels on the left. For cells 1-14 the merged image is double the size of the other images of these cells, whereas the merged image in 15-17* is the same scale as all the other panels. For full details see the text. The figure shows images of cells following 2 minutes fixation. The same patterns were seen if fixation time was extended to 30 minutes with the exception that the intensity diminished and the interphase nuclear spots and mitotic nuclear accumulation were no longer detected.