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Fig. 2. Schwann cells secrete PEDF. Media conditioned by human Schwann cells (20 µg protein, lane 2) and rat Schwann cells (20 µg protein, lane 3) were subjected to immunoblot analysis using an affinity purified antibody raised to PEDF-derived peptides. Recombinant human PEDF (20 ng, lane 1) was used as a positive control. M, molecular weight markers and their migration distance.