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Fig. 6. Western blot analysis of HDAC4 localization. Nuclear protein extracts (lanes 1-3) and cytoplasmic protein extracts (lanes 4-6) from C2.7 myoblasts transfected with pcHDAC4-Myc and with pFLAG/MEF2C (lanes 1,4) or with pFLAG/MEF2C{Delta}NLS (lanes 2,5) were subjected to western blot analysis with anti-Myc antibody. Nuclear protein extracts from cells transfected with pcHDAC4-Myc, alone (lane 7) or in combination with either pFLAG/MEF2C (lane 8) or pFLAG/MEF2C{Delta}NLS (lane 9), were immunoprecipitated with anti-FLAG antibody. Immunoprecipitates were subjected to western blot analysis with anti-Myc antibody. Lanes 3, 6 and 10 (C), are control lanes showing protein extracts from mock transfected cells.