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Fig. 10. Subcellular colocalization of Nesprin-1 in human tissues and in C2C12 differentiation. (A) Immunofluorescence of nesprin-1 in human tissues; aorta (I-III), heart (VI-VII), skeletal muscle (VIII-IX), spleen (X-XI) and leukocytes from peripheral blood (XII-XIII). Nesprins are shown in green, emerin and myosin in red, the nucleus in blue and rabbit IgG is a negative control (IV-V). (B) Immunolocalization of nesprin-1 in mouse C2C12 myoblasts (I-III) and myotubes (V-VII). Nesprin-1 is shown in green, emerin (IV and VIII), {alpha}-actin and myosin are shown in red.