Fig. 4. Predicted protein structure of nesprins-1 and -2. (A) Human nesprin protein domains generated by SMART and ProfileScan programmes. (B) Alignment of the 21 spectrin repeats of the nesprin-1 protein, showing homology domains within the three helices of each repeat. All G (orange) and P (yellow) residues are coloured. Other colouring is by conserved property in >55% of any column: uncoloured residues lack a sufficiently conserved property. Blue, hydrophobic residues; purple, negative residues, green, hydrophilic residues. (C) TMpred analysis showing the predicted hydrophobic TM domain in nesprin-1. (D) Alignment of the 60 C-terminal residues of nesprins showing homology with Drosophila Klarischt and C.elegans (unknown). Note the lack of sequence homology with C-terminal TM domains (shown in bold) found in other NE proteins, including emerin and myoferlin.