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Fig. 8. Subcellular colocalization of endogenous nesprin-1 in C2C12 myoblasts (A,C) and human VMSCs (B,D,E). Cytoskeletal {alpha}-actin (A), ER protein calnexin (B), and NE proteins, emerin (C), LAP1 (D), lamin A/C (E). Nesprins are shown in green, other markers in red (as indicated) and the nucleus in blue. Yellow in merged images indicates regions of colocalization.