Fig. 10. Localization of p95-C2 at the cell periphery. COS7 cells transfected for 20 hours with pFlag-p95-C2 were incubated for 3 hours without serum, before incubation for 10 minutes with medium containing 50 ng/ml of PDGF (B-E). After fixation, cells were immunostained for p95-C2 (A,B,D), and incubated with fluorescently-labelled phalloidin (C,E). Same fields are shown in B,C, and in D,E. Bar, 10 µm.