Fig. 2. The EMTOC appears towards the end of anaphase B. Wild-type cells (IH365) were cultured to mid-log phase in YES media at 25(C before processing for immunofluorescence microscopy. The spindle and cell length were measured. Because the cell structure shrinks back from the cell wall during preparation, the spindle length figures were calculated by multiplying measured spindle length by the length of the cell wall shell and dividing by the length of the shrunken cell structure.