Fig. 4. EMTOC formation does not require microtubules. nda3.1828 cells were manipulated by cold shock followed by treatment with 300 µg ml^–1 TBZ at 36°C, as described in the text before staining for tubulin (lower panels) and chromatin (DAPI/DIC) (upper panels). EMTOC formation (arrows) accompanied septation, as septation occurred in the absence of microtubules.