Fig. 1. F-actin binding ability of N-WASP/WAVE2. (a) Schematic structure of N-WASP and WAVE2. The number of amino acids from rat N-WASP or human WAVE2 is indicated. Abbreviations: A, acidic region; B, basic region; C, cofilin homology domain; CRIB, Cdc42/Rac interactive binding region; IQ, IQ motif; Pro, proline-rich region; SHD, SCAR/WAVE homology region; V, verprolin homology domain; WH1, WASP homology 1 domain. (b,c) Association of F-actin with the basic region of N-WASP (b) and WAVE2 (c). Wild-type protein and basic N-WASP (1 µM) or WAVE2 (0.3 µM) were mixed with F-actin (2 µM). The association with F-actin was assayed by co-sedimentation with F-actin after ultracentrifugation. The precipitates were analyzed by SDS-PAGE and Coomassie Brilliant Blue staining. The upper arrows indicate N-WASP or WAVE2, and the lower arrows indicate actin.