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Fig. 3. Basic region of N-WASP competed for actin filament binding. The association of full-length N-WASP with actin filaments in the presence or absence of protein fragments of the N-WASP binding region was analyzed by co-sedimentation assay. Association of full-length N-WASP with actin filaments was monitored with anti-N-WASP antibody. The concentrations of isolated N-WASP fragment are indicated.