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Fig. 2. Saturable association of Arl1 with the Golgi. Control CHO cells (A-C) were processed for double labeling to reveal endogenous Arl1 and GS28. Pooled Arl1-transfected (D-F) or Arl1-EGFP transiently transfected CHO cells (G-J) were fixed and double labeled with a monoclonal antibody against GS28 (E,H) and a limiting amount of Arl1 antibodies to reveal the exogenous expressed Arl1 (D,G). Arrows indicate the Golgi marked by GS28 (D,E). Bars: 10 µm.