Fig. 3. Golgi association of Arl1 depends on its N-terminal myristoylation. CHO cells were transiently transfected with constructs expressing Arl1(G2A)-EGFP in which the N-terminal myristoylation site Gly at position 2 was mutated into Ala, (A-C) or EGFP-Arl1 in which the myristoylation site was blocked by fusing Arl1 to the C-terminus of EGFP, (D-F). Cells were then processed to reveal the EGFP fusion protein as well as endogenous GS28. In contrast to Arl1-EGFP, these myristoylation-defective Arl1 mutants were not associated with the Golgi. Bars: 10 µm.