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Fig. 7. Arl1(Q71L) causes massive Brefeldin A (BFA)-resistant Golgi recruitment of AP-1 coat ({gamma}-adaptin). Pooled Arl1(Q71L) transfected CHO cells were treated with 10 µg/ml Brefeldin A for 0 minutes (A-C), 10 minutes (D-F) and 30 minutes (G-I) and then double labeled with a limiting amount of Arl1 antibody to reveal expressed Arl1(Q71L) and a monoclonal antibody against {gamma}-adaptin. Bars: 10 µm.