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Fig. 8. Arl1(Q71L) causes massive Brefeldin A (BFA)-resistant Golgi recruitment of Golgi ARFs. Pooled Arl1(Q71L)-transfected CHO cells were treated with 10 µg/ml Brefeldin A for 0 minutes (A-C), 5 minutes (D-F) and 60 minutes (G-I) and then double labeled with a limiting amount of Arl1 antibody to reveal expressed Arl1(Q71L) and with a monoclonal antibody against Golgi ARFs. Bars: 10 µm