Fig. 3. Silencing of lamin B1 and lamin B2. HeLa cells were transfected with lamin B1 siRNA (A,C), lamin B2 siRNA (E) or with luciferase siRNA (B,D,F). Cells were either double stained with lamin B1-specific antibody (A,B) and NuMA specific antibody (C,D) or stained for lamin B2 (E,F). (G) Western blot of cells transfected with lamin B1 (left) or luciferase (right) siRNA duplexes using lamin B1 antibody (top). The blot was stripped and re-probed with vimentin antibody to check for equal loading (bottom). Magnification 480x (A-F).