Fig. 5. Silencing of cytoplasmic actins in HeLa cells. (A) Phase-contrast of cells transfected with ß-actin siRNA duplex. Transfected cells show blebbing. (B) Western blot of cells transfected with ß-actin (left) or luciferase (right) siRNA duplexes using ß-actin antibody. The blot was stripped and re-probed with vimentin antibody to check for equal loading. (C) Phase-contrast of cells transfected with -actin siRNA duplex. Transfected cells show a blebbing phenotype similar to cells with ß-actin knockdown. Magnification 480x (A,C).